The severity of cascading global environmental, climate, economic, social and health crises is such that they sometimes seem insurmountable. ECLAC has therefore compiled a set of best practices, drawing from cases in Latin America and the Caribbean that can serve as models to promote comprehensive structural change and improve socioeconomic and environmental well-being. This study addresses challenges, opportunities and lessons learned as to how mainstreaming biodiversity in the agriculture, fisheries, forestry, financial, manufacturing, infrastructure and tourism sectors is a catalyst for the transition towards comprehensive development, in line with the 2030 Agenda, and a fundamental tool for the implementation of the new post-2020 global biodiversity framework. The cases, implemented at varying scales, in diverse ecosystems and with different approaches, show that the shift towards environmentally-friendly production and development patterns is under way in various sectors of the region and that initiatives can be replicated and scaled up.